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Resources
The
Southwestern PGA is currently producing the following resources
for the research community:
Microarray
expression data of mouse models of heart disease:
- Hypertrophic
Models: aortic banding, isoproterenol, hyperthyroidism and exercise
have all been investigated. Array hybridization data using the
muscle-specific array is available.
-
Macrophage analysis: array data (using the macrophage-specific
array) on peritoneal macrophages after LPS treatment is available.
- Endothelial
analysis: array data on LPS treatment followed by endothelial
isolation and analysis on the operon V2 array has been obtained
and will be shortly placed on line.
- Future
Analysis: Macrophage and endothelial data in the ApoE hypercholesterolemic
models will be obtained as soon as sufficient Tie2GFP/ApoE null
animals are available. We anticipate this will be completed
in 6 months using the Operon V3/MGH long oligo combined array.
Computational
Programs:
Programs
have been developed in the area of genetic analysis (microarrays,
exon mapping, primer prediction) and in prediction of mutations.
Skip: on the page listing the programs please divide up as to at
least these two categories or others.
Single
Nucleotide Polymorphisms:
A
list of the genes that have been sequenced, a table of the SNPs
discovered, and linkage to a description of the gene have been generated.
This is list is constantly being updated as new genes are sequenced.
Population
frequencies of SNPs and potential phenotype associations:
The
SNPs discovered in a small population (24-48) are then screened
in the DNA from the ~3500 member Dallas Heart Study population,
using the Sequenom high through put system. The frequency of particular
alleles by race are posted. Using the extensive clinical data on
the DHS population, any potential association of a SNP with a clinical
phenotype is noted.
Production of antibodies on request to human, mouse or rat
proteins:
Antibodies
are being produced by genetic immunization to proteins of interest
to the research community. No materials are required for the request
other than the sequence of the protein target.
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