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How to Make Antibodies using Genetic Immunization.
This slide show gives the viewer an overview of the principles of the
technique. Genetic immunization offers several advantages over traditional
approaches for generating antibodies. Most importantly it does not require any
protein (except a small amount of unpurified antigen to test the antibody).
Antigen genes are simply cloned into a plasmid, and delivered into an animal.
For detailed protocols contact Dr. R.S. Chambers ( ross.chambers@utsouthwestern.edu).
The procedure shown is the simplest version of the procedure. More advanced
versions developed by our laboratory are available (different genetic
immunization vectors, adjuvants and non-cloning methods for preparing the
antigen gene for immunization).
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