Banded Heart Microarray Data Set I

Downloads

• Entire Data set (Large file: 13,872, spots 3 arrays)
• Highly regulated data subsubset (>3 fold Regulation)

Background:  Aortic banding was performed as previously described (Rockman et al, PNAS 88:8277, 1991). Briefly, after general anesthesia and intubation, a small left-sternal thoracotomy was performed and aortic constriction accomplished by ligation of the aorta next to a 27 gauge needle, leaving a 0.4 mm lumen when the needle was removed. This approach produces a rapid increase in LV mass of approximately 35% after one week. Trans-ligation gradients were measured prior to sacrifice and hearts from animals with gradients of 60-70 mm Hg were used in these experiments. The heart was rapidly excised, the left ventricle was isolated by dissection and frozen in liquid nitrogen. Hearts were obtained after 1, 4 and 8 weeks of banding.

Microarrays Used: Mouse 13.8K cDNA spotted.

cDNA Libraries on Spotted Array:  

• UTSW:  Mouse cardiac and skeletal muscle
• INCYTE IMAGE : Stratagene fetal heart and Soares adult heart

Spotter: In-house Magna Spotter

Hybridization Type: RNA vs. RNA

Image Acquisition and Data Collection Hardware/Software:  Axon GenePix 4000A Scanner/GenePix Pro 3.0 analysis

Data Analysis/Normalization Software: MarC-V

Expression Profiling/Cluster Analysis Software: J-Express(B. Dysvik)/Cluster/Treeview (M. Eisen)